Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correction: Tang, L. et al., Effect of Oxygen Variation on High Cycle Fatigue Behavior of Ti-6Al-4V Titanium Alloy. <i>Materials</i> 2020, <i>13</i>, 3858.

Materials (Basel, Switzerland)·2020

Same author

Increased early activation of CD56dimCD16dim/- natural killer cells in immunological non-responders correlates with CD4+ T-cell recovery.

Chinese medical journal·2020

Same author

In situ experimental measurement of mercury by combining PGNAA and characteristic X-ray fluorescence.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2020

Same author

Tris (1,3-dichloro-2-propyl) phosphate exposure disrupts the gut microbiome and its associated metabolites in mice.

Environment international·2020

Same author

Genome Resource of <i>Sphingomonas carotinifaciens</i> L9-754<sup>T</sup>, an Endophyte Isolated From Leaf Tissues of <i>Jatropha curcas</i>.

Plant disease·2020

Same author

Heterozygous <i>PGM3</i> Variants Are Associated With Idiopathic Focal Epilepsy With Incomplete Penetrance.

Frontiers in genetics·2020

Search research articles

Related Experiment Video

Updated: May 19, 2026

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Elongation factor 1β' gene from Spodoptera exigua: characterization and function identification through RNA

Li-Na Zhao¹, Zi Qin¹, Ping Wei¹

¹Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.

International Journal of Molecular Sciences

|September 4, 2012

Summary

Elongation factor 1β

Keywords:

RNAi Spodoptera exigua cloning elongation factor expression pattern

More Related Videos

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Published on: October 13, 2014

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Related Experiment Videos

Last Updated: May 19, 2026

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Published on: October 13, 2014

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Area of Science:

Molecular Biology
Genetics
Insect Physiology

Background:

Elongation factors (EFs) regulate crucial translation processes across diverse organisms.
Elongation factor 1β' (EF-1β') plays a vital role in protein synthesis.
Understanding EF-1β' function in insects like Spodoptera exigua is essential for pest control and biological studies.

Purpose of the Study:

To characterize the elongation factor 1β' (SeEF-1β') from the insect Spodoptera exigua.
To investigate the expression patterns and functional significance of SeEF-1β' in S. exigua.

Main Methods:

cDNA cloning and sequence analysis of SeEF-1β'.
Northern blotting and RT-PCR to determine mRNA expression levels and tissue distribution.
RNA interference (RNAi) to assess the functional impact of SeEF-1β' suppression.

Main Results:

SeEF-1β' cDNA contains an open reading frame encoding a 223-amino acid protein (24.04 kDa, pI 4.53).
SeEF-1β' mRNA is expressed in various tissues (brain, epidermis, fat body, midgut, Malpighian tubules, ovary, tracheae) and developmental stages.
RNAi-mediated suppression of SeEF-1β' significantly reduced insect survival rates and affected other transcription factors.

Conclusions:

SeEF-1β' is a critical gene involved in transcription in Spodoptera exigua.
The study provides insights into the essential role of EF-1β' in insect development and survival.
Findings highlight SeEF-1β' as a potential target for insect pest management strategies.