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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Related Experiment Video

Updated: May 19, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Molecular imaging probe development: a chemistry perspective.

Donald D Nolting1, Michael L Nickels, Ning Guo

  • 1Vanderbilt University Institute of Imaging Science.

American Journal of Nuclear Medicine and Molecular Imaging
|September 4, 2012
PubMed
Summary
This summary is machine-generated.

Molecular imaging enables early disease detection using probes for positron emission tomography (PET), optical, and magnetic resonance imaging (MRI). This review covers chemical synthesis trends for these vital biomedical research tools.

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Area of Science:

  • Biomedical Research
  • Molecular Imaging
  • Diagnostic Tools

Background:

  • Molecular imaging is crucial for early disease detection (cancer, inflammation, neurodegenerative disorders).
  • It supports a healthcare paradigm focused on disease prediction and prevention.
  • Enables non-invasive, repeated, and continuous visualization of molecular events in vivo.

Purpose of the Study:

  • To review major trends in the chemical synthesis and development of molecular imaging probes.
  • To highlight the utility of probes in various biomedical research applications.
  • To discuss probes for positron emission tomography (PET), optical, and magnetic resonance imaging (MRI).

Main Methods:

  • Review of chemical synthesis strategies for PET, optical, and MRI probes.
  • Analysis of probe characteristics: compactness, robustness, and high-throughput production.
  • Discussion of probe applications in preclinical drug discovery and biomarker validation.

Main Results:

  • Identified key trends in the chemical synthesis of PET, optical, and MRI probes.
  • Highlighted the versatility of molecular imaging probes in research.
  • Demonstrated the potential for high-throughput production and application in drug discovery.

Conclusions:

  • Molecular imaging probes are essential for advancing biomedical research and early disease detection.
  • Continued development in chemical synthesis enhances probe capabilities.
  • These probes are valuable for drug discovery, safety evaluation, and biomarker validation.