Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PhyloNaP: a user-friendly database of phylogeny for natural product-producing enzymes.

Bioinformatics (Oxford, England)·2026
Same author

Optogenetic Stimulation of Novel Tph2-Cre Rats Advances Insight into Serotonin's Role in Locomotion, Reinforcement, and Compulsivity.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025
Same author

An analgesic pathway from parvocellular oxytocin neurons to the periaqueductal gray in rats.

Nature communications·2023
Same author

Effects of chess-based cognitive remediation training as therapy add-on in alcohol and tobacco use disorders: protocol of a randomised, controlled clinical fMRI trial.

BMJ open·2023
Same author

A Clathrin light chain A reporter mouse for in vivo imaging of endocytosis.

PloS one·2022
Same author

Altered PVN-to-CA2 hippocampal oxytocin pathway and reduced number of oxytocin-receptor expressing astrocytes in heart failure rats.

Journal of neuroendocrinology·2022

Related Experiment Video

Updated: May 19, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Conditional gene expression systems in the transgenic rat brain.

Kai Schönig1, Tillmann Weber, Ariana Frömmig

  • 1Department of Molecular Biology, Central Institute of Mental Health and Heidelberg University, Medical Faculty Mannheim, J5, 68159 Mannheim, Germany.

BMC Biology
|September 5, 2012
PubMed
Summary

Researchers developed new transgenic rats for precise control of gene expression in the brain. This breakthrough enables advanced studies in neuroscience using the Cre/loxP and Tet systems in rats.

More Related Videos

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
10:34

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Published on: September 17, 2010

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
11:03

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Published on: December 22, 2014

Related Experiment Videos

Last Updated: May 19, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
10:34

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

Published on: September 17, 2010

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
11:03

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Published on: December 22, 2014

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Conditional gene expression systems are crucial for studying gene function in vivo.
  • While common in mice, conditional transgenic systems are rare in rats, a key model organism in biomedical research, especially neuroscience.
  • Existing systems like Cre/loxP and Tet offer spatial, temporal, and reversible control of gene activity.

Purpose of the Study:

  • To address the scarcity of conditional transgenic systems in rats.
  • To establish and characterize CreERT2 and tTA transgenic rats for forebrain-specific gene expression control.
  • To develop novel rat reporter lines for Tet and Cre/loxP systems.

Main Methods:

  • Generation of forebrain-specific CreERT2 and tTA transgenic rats using the CaMKII alpha promoter.
  • Development of universal rat reporter lines compatible with both Tet and Cre/loxP systems.
  • Characterization of inducible and efficient reporter gene expression in forebrain neurons.

Main Results:

  • Successfully established and validated CreERT2 and tTA transgenic rats with forebrain-specific expression.
  • Developed and characterized new universal rat reporter lines for inducible gene expression.
  • Demonstrated efficient and inducible reporter gene expression in rat forebrain neurons.

Conclusions:

  • Conditional genetic manipulation in the rat brain is now feasible and practical.
  • The study outlines the advantages and limitations of Tet and Cre/loxP systems in the rat brain.
  • These new tools will significantly advance neuroscience research in rats.