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Updated: May 19, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
[Chromosome pairing in wheat-rye ABDR hybrids depends on the microsporogenesis pattern]
Abstract:
The character of chromosome pairing in meiocytes was studied in F1 wheat-rye Triticum aestivum L. x Secale cereale L. (ABDR, 4x = 28) hybrids with three types of chromosome behavior: reductional, equational, and equational + reductional. A high variation of the frequencies of bivalents and ring univalents was observed in meiocytes with the reductional or equational + reductional type of chromosome behavior. The type of chromosome division was found to affect the bivalent and ring univalent frequencies. Chromosome pairing occurred in 10.28% of meiocytes with the reductional chromosome behavior, 0.93% of meiocytes with the equational chromosome behavior, and 10.81% of meiocytes with the equational + reductional chromosome behavior. On average, 0.13 bivalents per cell formed in meiocytes of the hybrid population. C-banding and genomic in situ hybridization (GISH) showed that both rye and wheat chromosomes produced ring univalents. The role of the Ph genes in regulating the bivalent formation in meiocytes with different types of chromosome behavior is discussed.
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