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Dried matrix on paper disks: the next generation DBS microsampling technique for managing the hematocrit effect in
Roland Jw Meesters1, Jamie Zhang, Nick A van Huizen
1Universidad de los Andes, Faculty of Sciences, Department of Chemistry, Carrera 1 No 18A-10, Bogota, Colombia. rj.meesters@uniandes.edu.co
Background:
The hematocrit effect is a hurdle for successful introduction of the dried blood spot (DBS) in a regulated environment. Recently, attempts were taken to overcome the hematocrit effect by whole-cut DBS analysis. This paper presents the next-generation whole-cut DBS; dried matrix on paper disks (DMPD).
Results:
DMPD eliminated the hematocrit effect and demonstrated better accuracy and precision than regular DBS with partial punching. Observed accuracy and precision were 6.0 and 2.3% for DMPD, respectively, and -10.4 and 17.1%, for DBS, respectively.
Conclusion:
The DMPD technique performed better than regular DBS by eliminating the hematocrit effect related blood volume bias. Although this effect was not observed with DMPD, a systematic error of 6.0% was detected and further technical development of DMPD could improve the performance.
