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Updated: May 19, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
Published on: December 1, 2017
The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard
Matthijn C Hesselman1, Jasper J Koehorst1, Thijs Slijkhuis1
1iGEM Wageningen UR, Wageningen University, Wageningen, The Netherlands.
Abstract:
Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biological Parts harbours such a repository of standardized parts, and thereby facilitates the combination of complex molecular modules to novel genetic circuits and devices. However, since finding the best parts for a pre-determined genetic design can be time consuming, we devised the Constructor, a web tool that recommends the smallest number of cloning steps for pre-designed circuits, and implements user-defined quality checks.We present the Constructor ( http://www.systemsbiology.nl/the_constructor) as a constructive web tool that simplifies the in silico assembly of pre-designed gene circuitries from standard parts, reducing both planning and subsequent cloning time.
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