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Related Experiment Video

Updated: May 19, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

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G-quadruplex DNA aptamers for zeatin recognizing.

Cui Qi1, Tao Bing, Hongcheng Mei

  • 1Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Biosensors & Bioelectronics
|September 6, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed specific oligonucleotide ligands (aptamers) for zeatins, crucial plant hormones. These aptamers enable a sensitive graphene oxide aptasensor for detecting zeatins in biological research and chemical analysis.

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Area of Science:

  • Plant biology
  • Biochemistry
  • Analytical chemistry

Background:

  • Zeatins are key plant hormones (cytokinins) regulating growth and development.
  • Specific detection tools for zeatins are vital for plant research.

Purpose of the Study:

  • To develop highly specific oligonucleotide ligands (aptamers) for zeatins.
  • To create a sensitive aptasensor for zeatin detection.

Main Methods:

  • Generation and optimization of aptamers against trans-zeatin.
  • Characterization of aptamer binding affinity and specificity.
  • Development of a graphene oxide (GO)-based fluorescence turn-on aptasensor.

Main Results:

  • Optimized aptamers showed high affinity for trans-zeatin and trans-zeatin riboside (Kd=3-5 μM).
  • Aptamers exhibited lower affinity for cis-zeatin and dihydrozeatin (Kd=27-30 μM).
  • The GO aptasensor demonstrated specific zeatin recognition without response to adenine derivatives.

Conclusions:

  • Developed aptamers possess high specificity and affinity for zeatins.
  • The aptasensor offers a promising tool for zeatin detection in biological samples.
  • These aptamers have significant potential for plant science research and chemical analysis.