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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
The grapevine expression atlas reveals a deep transcriptome shift driving the entire plant into a maturation program
Marianna Fasoli1, Silvia Dal Santo, Sara Zenoni
1Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy.
The Plant Cell
|September 6, 2012
Summary
This study created a grapevine gene expression map, revealing distinct transcriptomes for specialized tissues and a major shift from green to woody stages. This maturation reprogramming is unique to perennial woody plants.
Area of Science:
- Plant biology
- Genomics
- Transcriptomics
Background:
- Grapevine (Vitis vinifera) is a vital perennial woody crop.
- Understanding its gene expression patterns is crucial for agricultural and biological insights.
- Previous studies have not comprehensively mapped the genome-wide transcriptomic landscape across diverse tissues and developmental stages.
Purpose of the Study:
- To develop a comprehensive genome-wide transcriptomic atlas of grapevine.
- To identify distinct transcriptomic profiles of various tissues and developmental stages.
- To investigate the transcriptomic reprogramming associated with maturation in perennial woody plants.
Main Methods:
- Collected 54 grapevine samples representing green and woody tissues, different developmental stages, and specialized tissues (pollen, senescent leaves).
- Utilized microarray and RNA-sequencing (RNA-seq) for transcriptomic analysis.
- Applied correlation analysis, O2PLS-DA, and biclustering for statistical analysis of gene expression data.
Main Results:
- The atlas captured expression for approximately 91% of predicted grapevine genes.
- Pollen and senescent leaves exhibited unique transcriptomes reflecting specialized functions.
- Samples primarily clustered into vegetative/green and mature/woody categories, indicating a fundamental transcriptomic shift during maturation.
- Gene coexpression analysis revealed reiterative coactivation of pathways during woody development, involving coregulation of gene clusters and codon preference.
Conclusions:
- Grapevine maturation involves a global transcriptomic reprogramming not observed in herbaceous annual species.
- This maturation process may be a defining characteristic of perennial woody plants.
- The developed transcriptomic atlas provides a valuable resource for grapevine research.
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