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Related Concept Videos

Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

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Related Experiment Video

Updated: May 18, 2026

Fast and Accurate Exhaled Breath Ammonia Measurement
06:27

Fast and Accurate Exhaled Breath Ammonia Measurement

Published on: June 11, 2014

Developing an amperometric hydrogen peroxide sensor for an exhaled breath analysis system.

Justyna Wiedemair1, Henriëtte D S van Dorp, Wouter Olthuis

  • 1MESA+ Institute for Nanotechnology, University of Twente, The Netherlands. j.wiedemair@utwente.nl

Electrophoresis
|September 6, 2012
PubMed
Summary
This summary is machine-generated.

We developed a novel chip-integrated amperometric sensor for detecting gaseous hydrogen peroxide (H₂O₂). This sensor achieves a low limit of detection, paving the way for breath analysis applications.

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Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

Related Experiment Videos

Last Updated: May 18, 2026

Fast and Accurate Exhaled Breath Ammonia Measurement
06:27

Fast and Accurate Exhaled Breath Ammonia Measurement

Published on: June 11, 2014

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

Area of Science:

  • Electrochemistry
  • Chemical Sensing
  • Microfabrication

Background:

  • Hydrogen peroxide (H₂O₂) is a key biomarker in exhaled breath.
  • Accurate detection of gaseous H₂O₂ is challenging but crucial for non-invasive diagnostics.
  • Existing sensing methods often lack sensitivity or require complex sample handling.

Purpose of the Study:

  • To develop and characterize a chip-integrated amperometric sensor for gaseous H₂O₂ detection.
  • To optimize sensor performance, including limit of detection and linearity.
  • To demonstrate the sensor's capability for real-time H₂O₂ monitoring.

Main Methods:

  • Microfabrication of electrode chips.
  • Electrochemical characterization using cyclic voltammetry and amperometry.
  • Optimization of electrolyte composition and electrode pretreatment.
  • Gas-phase H₂O₂ detection using agarose-coated electrode chips in static and flow-through setups.

Main Results:

  • Successful electrochemical detection of H₂O₂ in aqueous and gaseous phases.
  • Electrolyte composition significantly impacts signal response.
  • Electrochemical pretreatment of platinum electrodes enhances sensitivity and linearity.
  • A limit of detection of approximately 42 ppb for gaseous H₂O₂ was achieved.

Conclusions:

  • The developed chip-integrated amperometric sensor is effective for gaseous H₂O₂ detection.
  • Sensor performance is tunable through electrochemical pretreatment and electrolyte optimization.
  • The technology shows promise for analyzing H₂O₂ in exhaled breath condensate.