Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microfluidic patch integrated with cobalt oxide/cobalt phosphate nanozyme for electrochemical lactate sensing at neutral pH.

Talanta·2026
Same author

A Polydopamine-Based Molecularly Imprinted Electrochemical Sensor for Fentanyl Determination.

ACS omega·2025
Same author

Rapid assembly of mixed thiols for toll-like receptor-based electrochemical pathogen sensing.

Analytical methods : advancing methods and applications·2024
Same author

Integration of complementary split-ring resonators into digital microfluidics for manipulation and direct sensing of droplet composition.

Lab on a chip·2024
Same author

Electrochemical Determination of Fentanyl Using Carbon Nanofiber-Modified Electrodes.

ACS omega·2024
Same author

Celebrating the 30th anniversary of a pioneering microfluidics paper.

Lab on a chip·2023
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Integrated electrokinetic sample fractionation and solid-phase extraction in microfluidic devices.

Zhen Wang1, Abebaw B Jemere, D Jed Harrison

  • 1Department of Chemistry, University of Alberta, Edmonton, AB, Canada.

Electrophoresis
|September 6, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic device for proteomics that fractionates, collects, and preconcentrates samples. It enables efficient protein analysis with a preconcentration factor of 30, advancing automated proteomics research.

More Related Videos

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device
06:28

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Published on: February 2, 2024

Related Experiment Videos

Last Updated: May 18, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device
06:28

Single Step Isolation of Extracellular Vesicles from Large-Volume Samples with a Bifurcated A4F Microfluidic Device

Published on: February 2, 2024

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Microfluidics

Background:

  • Proteomics research requires efficient sample preparation techniques.
  • Existing methods for sample fractionation, collection, and preconcentration can be time-consuming and complex.
  • Microfluidic platforms offer potential for miniaturized and automated analytical processes.

Purpose of the Study:

  • To describe a novel microfluidic device for "in space" sample fractionation, collection, and preconcentration.
  • To detail the device design, fabrication, and performance optimization for electrokinetic sample handling.
  • To demonstrate the device's utility in proteomics research.

Main Methods:

  • Development of a microfluidic device with a fractionation channel and 36 collection channels.
  • Simultaneous photolytic fabrication of 36 monolithic polymer columns for solid-phase extraction (SPE).
  • Utilized a butyl methacrylate-based polymer monolith with an ionizable monomer for SPE and electroosmotic flow.

Main Results:

  • Achieved efficient electrokinetic fractionation of protein samples into 36 distinct collection channels without cross-contamination.
  • Demonstrated reproducible performance of monolithic columns with a preconcentration factor of 30 achieved in 2 minutes.
  • Optimized device design and fabrication conditions for effective sample handling.

Conclusions:

  • The developed microfluidic device successfully integrates fractionation, collection, and preconcentration.
  • This platform is highly suitable for automated or continuous operation in proteomics.
  • The device offers a significant advancement for high-throughput and sensitive protein analysis.