Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regulatory volume increase in Ehrlich ascites tumor cells.

C Levinson1

  • 1Department of Physiology, University of Texas Health Science Center, San Antonio 78284.

Biochimica Et Biophysica Acta
|January 15, 1990
PubMed
Summary

Cell volume regulation in Ehrlich ascites tumor cells involves the Na+/K+ pump and a (2Cl- + K+ + Na+) cotransport system. Chloride transport is key to restoring normal cell volume and concentration.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BRCA1 screening in a woman with breast cancer: a patient's perspective with commentary.

Women's health issues : official publication of the Jacobs Institute of Women's Health·2001
Same author

Datapoints: employer-based mental health benefits: implications for mental health parity.

Psychiatric services (Washington, D.C.)·2000
Same author

Characterization of chloride efflux from GT1-7 neurons: lack of effect of ethanol on GABAA response.

Brain research·1998
Same author

Chloride accumulation in freshly isolated Ehrlich ascites tumor cells: the role of the Na/K/2Cl cotransporter.

Biochimica et biophysica acta·1996
Same author

Regulatory volume decrease in Ehrlich ascites tumor cells is not mediated by a rise in intracellular calcium.

Biochimica et biophysica acta·1993
Same author

Regulatory volume increase in Ehrlich ascites tumor cells is mediated by the 1Na:1K:2Cl cotransport system.

The Journal of membrane biology·1992

Area of Science:

  • Cell Biology
  • Ion Transport
  • Tumor Physiology

Background:

  • Ehrlich ascites tumor cells experience volume changes due to ion imbalances.
  • The Na+/K+ pump and cotransport systems are involved in maintaining ionic homeostasis.

Purpose of the Study:

  • To investigate the role of ion transport in restoring cell volume.
  • To determine the specific contribution of chloride transport to cell volume regulation.

Main Methods:

  • Analysis of ionic concentrations and cell volume in Ehrlich ascites tumor cells.
  • Investigation of the Na+/K+ pump and (2Cl- + K+ + Na+) cotransport system activity.
  • Correlation of intracellular chloride levels with cell volume restoration.

Main Results:

  • Cell volume restoration correlates with increased intracellular chloride.
  • Chloride transport, primarily via the cotransport system, dictates steady-state intracellular chloride concentration.
  • Transport cessation occurs when ion chemical potentials reach equilibrium, establishing a steady state for chloride.

Conclusions:

  • Chloride transport plays a critical role in regulating cell volume in Ehrlich ascites tumor cells.
  • The (2Cl- + K+ + Na+) cotransport system is the primary mediator of net chloride uptake.
  • Cell volume regulation is intrinsically linked to chloride homeostasis and transport dynamics.

Related Experiment Videos