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Updated: May 18, 2026
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Protocol for Long Duration Whole Body Hyperthermia in Mice
Published on: August 25, 2012
Protocol for long duration whole body hyperthermia in mice
Vikas Duhan1, Neha Joshi, P Nagarajan
1Product Development Cell, National Institute of Immunology.
Journal of Visualized Experiments : Jove
|September 7, 2012
Summary
Researchers developed a reliable whole-body hyperthermia protocol for mice, simulating fever for cancer research. This method ensures uniform heating, crucial for studying hyperthermia
Area of Science:
- Oncology
- Physiology
Background:
- Hyperthermia, an increase in core body temperature, is investigated as an adjuvant cancer therapy.
- Effective tumor regression via hyperthermia necessitates reliable and uniform heating protocols.
Purpose of the Study:
- To develop and present a standardized whole-body hyperthermia protocol for mice.
- To simulate fever-like conditions in mice for cancer research applications.
Main Methods:
- Mice underwent cycles of 90-minute hyperthermia followed by 15-minute rest periods.
- Immobilization and normal saline administration were used during initial cycles to prevent temperature spikes and dehydration.
- The protocol utilized 8-12 week old BALB/Cj female mice.
Main Results:
- The developed protocol successfully simulates fever-like conditions in mice.
- The protocol can maintain simulated fever for 12-24 hours.
- Measures were effective in preventing adverse effects like extreme temperature spikes and dehydration.
Conclusions:
- A reproducible whole-body hyperthermia protocol for mice has been established.
- This protocol provides a valuable tool for investigating hyperthermia's role in cancer biology.
