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Human immunodeficiency virus cultured from bone. Implications for transplantation
B E Buck1, L Resnick, S M Shah
1Department of Surgery, University of Miami School of Medicine, FL 33101.
Clinical Orthopaedics and Related Research
|February 1, 1990
Summary
Human immunodeficiency virus (HIV) can be present in bone tissue. Freezing bone may reduce the risk of HIV transmission through bone allografts, complementing existing screening methods.
Area of Science:
- Virology
- Infectious Diseases
- Tissue Banking
Background:
- Human immunodeficiency virus (HIV) is a significant public health concern.
- Bone allografts are used in various surgical procedures.
- Ensuring the safety of allografts is crucial to prevent disease transmission.
Purpose of the Study:
- To investigate the presence of HIV in bone tissue.
- To evaluate the efficacy of bone processing methods in inactivating HIV.
- To assess the safety of bone allografts for transplantation.
Main Methods:
- Virologic culture methods were used to detect HIV in bone specimens.
- Bone specimens underwent processing steps including freezing, washing, and freeze-drying.
- Viral yield was assessed after each processing step.
Main Results:
- HIV was detected in bone specimens, confirming its presence in this tissue.
- Freezing reduced the viral load in some specimens.
- Further processing steps like washing and freeze-drying did not significantly alter viral yield in specimens that remained positive after freezing.
Conclusions:
- Bone tissue can harbor HIV.
- Freezing is a potential step to reduce residual HIV risk in bone allografts.
- Current screening and testing methods remain the primary safeguards against HIV transmission via bone allografts.