The endoscopic-assisted trephination approach for repair of frontal sinus cerebrospinal fluid leaks

This protocol details a minimally invasive endoscopic technique for the removal of third ventricular colloid cysts. It provides a comprehensive overview of preoperative preparations, surgical steps, and postoperative outcomes, emphasizing reduced recovery time, minimal complications, and total cyst removal. This approach is a safe and effective alternative to traditional...

The present protocol describes the steps and key points of lumbar endoscopic unilateral laminotomy for bilateral decompression for the treatment of degenerative lumbar spinal...

Intrathecally applied fluorescein is used to achieve intraoperative visualization of CSF leaks. This protocol describes a lumbar puncture, the application of 5% fluorescein, and intraoperative visualization using a fully digital...

A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous...

Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....

Centrifuge the sample to pellet cellular debris and collect the...