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Development of the Fc-III tagged protein expression system for protein purification and detection

Shan Feng1, Enbing Tian, Lei Zhang

  • 1School of Life Sciences, Tsinghua University, Beijing, China.

Plos One
|September 7, 2012
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...