Related Experiment Video
Updated: May 18, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The complete mitochondrial genome of Percocypris pingi (Teleostei, Cypriniformes)
Yanping Li1, Jinjin Wang, Zuogang Peng
1The Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Beibei, Chongqing 400715, P R China.
Abstract:
Percocypris pingi is an endemic and economic fish species only found in the upper Yangtze River basin in China. It has become endangered in recent years due to overfishing and/or dam construction. However, the available genetic data are still scarce for this species. Here, we sequenced the complete mitochondrial genome sequence of P. pingi using long polymerase chain reactions. The complete mitogenome sequence has 16,586 bp and contains the usual 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA (tRNA) genes, and 1 control region, the gene composition and order of which are similar to most of other vertebrates. Most mitochondrial genes except ND6 and eight tRNAs are encoded on the heavy strand. The overall base composition of the heavy strand is 30.9% A, 25.7% T, 26.6% C, and 16.8% G with a slight AT bias of 56.6%. There are seven regions of gene overlaps totaling 23 bp and 11 intergenic spacer regions totaling 35 bp. Combined with the COI barcoding region sequences of other 25 cyprinids, the phylogenetic position of P. pingi was estimated using neighbor-joining method. The results showed that P. pingi had a close phylogenetic relationship with the species from genus Schizothorax. This mitogenome sequence data of P. pingi would provide the fundamental genetic data for further conservation genetic studies for this endangered fish species.
Related Concept Videos
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Animal Mitochondrial Genetics
Export of Mitochondrial and Chloroplast Genes
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Bacterial Phylum Tenericutes

