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Continuous-flow biomolecule concentration and detection in a slanted nanofilter array.

Lih Feng Cheow1, Hansen Bow, Jongyoon Han

  • 1Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Lab on a Chip
|September 8, 2012
PubMed
Summary

We developed a herringbone nanofilter array (HNA) for continuous-flow concentration and detection of biomolecules. This microfluidic device enables sensitive detection of disease markers like C-reactive protein (CRP) for point-of-care diagnostics.

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Area of Science:

  • Microfluidics
  • Biomolecular Engineering
  • Nanotechnology

Background:

  • Microfluidic devices offer potential for point-of-care diagnostics.
  • Efficient concentration and detection of biomolecules are crucial for sensitive assays.
  • Existing batch separation techniques can be time-consuming and complex.

Purpose of the Study:

  • To demonstrate continuous-flow biomolecule concentration and detection using a novel microfabricated structure.
  • To develop a method for discriminating analytes based on size and concentration.
  • To showcase the application of this technology in interrogating biomolecular interactions and performing homogeneous immunoassays.

Main Methods:

  • Fabrication of a microfluidic herringbone nanofilter array (HNA) with slanted sieving structures.
  • Utilizing fluorescent probes to measure analyte concentration and size discrimination.
  • Interrogating biomolecular interactions such as protein-DNA, protein-protein, and antibody-antigen binding.
  • Developing a homogeneous immunoassay for human C-reactive protein (CRP) detection.

Main Results:

  • Successful continuous-flow concentration and detection of biomolecules were achieved.
  • The HNA structure enabled size discrimination of different analytes.
  • Various biomolecular interactions were successfully interrogated.
  • A sensitive homogeneous immunoassay for CRP detection at clinically relevant concentrations was demonstrated.

Conclusions:

  • The herringbone nanofilter array (HNA) is an effective platform for continuous-flow biomolecule concentration and detection.
  • This technology offers advantages in signal amplification and ease of integration for point-of-care diagnostic platforms.
  • The HNA enables sensitive detection of disease biomarkers, facilitating rapid diagnostics.