Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transgenic Organisms00:53

Transgenic Organisms

Overview
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Venetoclax in combination with cytarabine with or without idarubicin or azacitidine in children, adolescents, and young adults with relapsed or refractory acute myeloid leukaemia (VENAML): a multicentre, phase 1 expansion study.

The Lancet. Haematology·2026
Same author

Quantifying interpretive contributions to analytical variability in clinical flow cytometry.

Cytometry. Part B, Clinical cytometry·2026
Same author

Machine learning-based prediction of nasopharyngeal carcinoma risk: a clinical approach.

Frontiers in immunology·2025
Same author

Pediatric T-Lymphoblastic Leukemia With Aberrant B-Cell Marker Expression: A Potential Role for Targeted Therapy.

EJHaem·2025
Same author

A Nomogram for Predicting Lung Metastasis in Papillary Thyroid Cancer Patients Aged Less Than 55 Years.

Frontiers in endocrinology·2025
Same author

Clonal Evolution of Pediatric Acute Myeloid Leukemia and Its Contribution to Disease Relapse.

medRxiv : the preprint server for health sciences·2025

Related Experiment Video

Updated: May 18, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Xenopus transgenics: methods using transposons.

Clair M Kelley1, Donald A Yergeau, Haiqing Zhu

  • 1Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 8, 2012
PubMed
Summary

Efficiently generate transgenic Xenopus animals using DNA transposon systems. This method involves co-injecting transposon DNA and transposase mRNA into early embryos for stable genomic integration.

More Related Videos

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Related Experiment Videos

Last Updated: May 18, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Transgenic animals are crucial for genetic research.
  • Efficient genome modification is needed for Xenopus studies.

Purpose of the Study:

  • To present a DNA transposon system for efficient Xenopus genome modification.
  • To enable the generation of transgenic Xenopus.

Main Methods:

  • Co-injection of DNA transposon substrate and transposase mRNA into one-cell stage Xenopus embryos.
  • Utilizing the cellular machinery for mRNA translation and transposase production.
  • Transposase-mediated excision and genomic integration of the DNA transposon.

Main Results:

  • Successful generation of transgenic Xenopus embryos.
  • Stable integration of the transposon into the host genome.
  • Demonstration of efficient Xenopus genome modification.

Conclusions:

  • DNA transposon systems provide an efficient method for creating transgenic Xenopus.
  • This technique facilitates genetic strategies in Xenopus research.
  • The described method offers a robust tool for Xenopus transgenesis.