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RIP140 is associated with subclinical inflammation in type 2 diabetic patients
1Department of Endocrinology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Aims:
To evaluate the expression level of RIP140 (receptor interaction protein 140) and its correlation with inflammatory cytokine production and free fatty acids (FFAs) in type 2 diabetes.
Methods:
Plasma and peripheral blood mononuclear cells (PBMCs) were collected from 24 diabetic and 30 healthy individuals. The levels of FFAs, TC, TG, HDL-C, LDL-C, FIN, and FBG were measured. The insulin resistance index was calculated using the homeostasis model assessment (HOMA). Additionally, PBMCs from control group were cultured alone or with 500 μmol/L palmitic acid (PA). Levels of RIP140 TNF-α, and IL-6 in PBMCs were analyzed using real-time RT-PCR, Western blots and ELISA. The relationship between RIP140 and other variables was performed using SPSS 11.5 software.
Results:
TG, LDL-C, FIN, FBG, HOMA, and HDL-C were significantly different between diabetic patients and the control group. Levels of RIP140, TNF-α, and IL-6 were higher in the diabetic group compared to control. RIP140 expression was positively correlated with FFAs, HDL-c, TNF-α, IL-6, FIN, FBG, and HOMA. Finally, 500 μmol/L PA treatment increased RIP140 expression and the secretion of inflammatory cytokines in cultured control PBMCs.
Conclusions:
Increased RIP140 level may be closely associated with inflammation and disorder of lipid and glucose metabolism in diabetic patients.
Insights
Receptor interaction protein 140 (RIP140) levels are elevated in type 2 diabetes, correlating with inflammation and metabolic dysfunction. Increased RIP140 may contribute to the pathogenesis of diabetic complications.
Area of Science:
- Endocrinology and Metabolism
- Molecular Biology
- Immunology
Background:
- Type 2 diabetes is characterized by insulin resistance and chronic inflammation.
- Receptor interaction protein 140 (RIP140) is a nuclear receptor co-regulator implicated in metabolic processes.
- The role of RIP140 in the inflammatory and metabolic derangements of type 2 diabetes requires further elucidation.
Purpose of the Study:
- To investigate the expression of RIP140 in individuals with type 2 diabetes.
- To examine the correlation between RIP140 levels, inflammatory cytokines, and free fatty acids (FFAs) in type 2 diabetes.
- To assess the effect of palmitic acid on RIP140 expression and cytokine production in vitro.
Main Methods:
- Peripheral blood mononuclear cells (PBMCs) and plasma were obtained from 24 type 2 diabetic patients and 30 healthy controls.
- Levels of RIP140, TNF-α, and IL-6 were quantified in PBMCs using real-time RT-PCR, Western blot, and ELISA.
- Free fatty acids (FFAs), lipid profiles, fasting glucose (FBG), and fasting insulin (FIN) were measured; insulin resistance was assessed via HOMA.
Main Results:
- Diabetic patients exhibited significantly higher levels of RIP140, TNF-α, and IL-6 compared to controls.
- RIP140 expression positively correlated with FFAs, HDL-c, TNF-α, IL-6, FIN, FBG, and HOMA.
- Palmitic acid treatment in control PBMCs upregulated RIP140 and augmented inflammatory cytokine secretion.
Conclusions:
- Elevated RIP140 expression is strongly associated with inflammation and dysregulated lipid and glucose metabolism in type 2 diabetes.
- RIP140 may play a significant role in the pathophysiology of type 2 diabetes.
- Targeting RIP140 could offer a potential therapeutic strategy for managing diabetic complications.
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