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Crystallization and preliminary X-ray diffraction results of snowdrop (Galanthus nivalis) lectin
C S Wright1, H Kaku, I J Goldstein
1Department of Biochemistry and Molecular Biophysics, MCV/VCU, MCV Station, Richmond 23298-0001.
The Journal of Biological Chemistry
|January 25, 1990
Abstract:
Well-ordered single crystals have been grown for a mannose-specific lectin from snowdrop (Galanthus nivalis) bulbs in the presence of methyl-alpha-D-mannoside. The space group symmetry is consistent with the orthorhombic space group P2(1)2(1)2(1). The unit cell parameters are a = 140.0 A, b = 64.7 A, c = 62.0 A. The asymmetric unit can accommodate a tetramer. The functional molecule (50,000 daltons) consists of four identical subunits and is highly specific for alpha 1,3-linked mannose oligosaccharides.