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Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Updated: May 1, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Methods for cancer epigenome analysis.

Raman P Nagarajan¹, Shaun D Fouse, Robert J A Bell

¹University of California, San Francisco, CA, USA.

Advances in Experimental Medicine and Biology

|September 8, 2012

Summary

This summary is machine-generated.

Accurate detection of epimutations, including DNA methylation and histone modifications, is vital for understanding cancer. Advanced sequencing technologies enable comprehensive epigenome analysis in tumors, aiding in cancer research and treatment.

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Methyl-binding DNA capture Sequencing for Patient Tissues

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Published on: October 31, 2016

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Area of Science:

Epigenetics and Cancer Biology
Molecular Pathogenesis of Tumors

Background:

Epimutations, particularly DNA methylation alterations, are functionally important and clinically relevant in cancer.
New epigenetic marks and their roles, such as altered 5-hydroxymethylcytosine and histone modifications, are continually being discovered.
Understanding epimutations is crucial for deciphering cancer's molecular basis.

Purpose of the Study:

To highlight the importance of accurate epimutation detection in tumor cells.
To review current and emerging epigenome analysis methods for cancer research.
To emphasize the clinical relevance of epigenetic alterations in cancer.

Main Methods:

Next-generation sequencing (NGS) for whole-genome DNA methylation and hydroxymethylation profiling.
Chromatin immunoprecipitation with sequencing (ChIP-seq) for genome-wide mapping of histone modifications, open chromatin, and transcription factor binding sites.
Development of computational tools for epigenome data interpretation.

Main Results:

NGS enables increasingly inexpensive and quantitative whole-genome profiling of DNA and hydroxymethylation.
ChIP-seq allows for genome-wide mapping of histone modifications and other epigenetic marks.
Computational tools are advancing to facilitate the interpretation of complex epigenome profiling data.

Conclusions:

Accurate detection and analysis of epimutations are essential for understanding cancer pathogenesis.
Advances in epigenome analysis technologies are providing deeper insights into cancer biology.
Epigenetic alterations play significant roles in tumor etiology, response to therapy, and progression.