Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacologic activity, safety, and preliminary efficacy of GEN3009, a CD37-targeting DuoHexaBody, in relapsed or refractory B-cell non-Hodgkin's lymphoma.

Journal for immunotherapy of cancer·2026
Same author

Retargeted adenoviruses for local IgA and CD47 blocker production as a novel cancer therapy.

EMBO molecular medicine·2026
Same author

Preclinical Comparison of Two CD3xB7H4 Bispecific Antibodies for Solid Tumor Therapy Reveals CD3 Affinity-Dependent Potency and Tolerability Tradeoffs.

Molecular cancer therapeutics·2026
Same author

Myeloid cell-mediated killing of B-ALL by CD38 and CD20 IgA antibody variants is enhanced by CD47/SIRPα interference.

Blood neoplasia·2026
Same author

Safety and Efficacy of HexaBody-DR5/DR5 in Malignant Solid Tumors: Phase 1/2a Results and Translational Insights Into Observed Hepatotoxicity.

Clinical and translational science·2026
Same author

Reply to the correspondence on 'Development of bispecific antibodies that allow targeted local complement inhibition on rheumatoid arthritis related antigens'.

Annals of the rheumatic diseases·2026

Related Experiment Video

Updated: May 18, 2026

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

Crosstalk between human IgG isotypes and murine effector cells.

Marije B Overdijk1, Sandra Verploegen, Antonio Ortiz Buijsse

  • 1Genmab, 3584 CM, Utrecht, The Netherlands.

Journal of Immunology (Baltimore, Md. : 1950)
|September 8, 2012
PubMed
Summary

Human IgG (hIgG) isotypes show varied interactions with mouse Fc receptors (mFcγRs) and effector cells. Understanding this crosstalk is crucial for interpreting preclinical efficacy studies of human antibodies in mouse models.

More Related Videos

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

Related Experiment Videos

Last Updated: May 18, 2026

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

Area of Science:

  • Immunology
  • Pharmacology
  • Biotechnology

Background:

  • Human therapeutic antibodies (Abs) are engineered for reduced immunogenicity and optimal human immune system interaction.
  • Humanization of Abs complicates preclinical efficacy studies in mouse models due to incomplete understanding of murine effector cell activation by human IgG (hIgG).

Purpose of the Study:

  • To investigate the interactions between human IgG isotypes and mouse Fc gamma receptors (mFcγRs) and murine effector cells.
  • To compare the efficacy of different hIgG isotypes in preclinical mouse models.

Main Methods:

  • Development of human and mouse isotype variants of Abs targeting EGFR and CD20.
  • Analysis of mFcγR binding affinities and induction of Ab-dependent cellular cytotoxicity (ADCC) and Ab-dependent cellular phagocytosis.
  • Evaluation of hIgG isotype activity in subcutaneous (s.c.) and intravenous (i.v.) tumor xenograft models.

Main Results:

  • hIgG1 and hIgG3 bound to all tested mFcγRs, with hIgG3 showing higher affinity.
  • hIgG1 demonstrated greater potency in inducing ADCC and phagocytosis compared to hIgG3.
  • hIgG4 showed activity in the murine system, unlike its inert human phenotype.
  • hIgG2 bound to specific mFcγRs and induced potent ADCC but weak phagocytosis.
  • hIgG1 was confirmed as the most potent human isotype in mouse xenograft models.

Conclusions:

  • Distinct human IgG isotypes exhibit differential engagement with murine FcγRs and effector cells.
  • These findings improve the interpretation of human antibody efficacy studies in mouse models.
  • hIgG1 emerges as the most effective isotype for preclinical evaluation in mouse models.