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High-Density Lipoprotein-Specific Phospholipid Efflux Assay

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Published on: September 30, 2025

Plasma Lp-PLA(2) mass and apoB-lipoproteins that carry Lp-PLA(2) decrease after sodium.

Alexander Constantinides¹, Michiel N Kerstens, Bert D Dikkeschei

¹Department of Endocrinology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.

European Journal of Clinical Investigation

|September 11, 2012

Summary

A short-term sodium challenge significantly reduced lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) mass and apolipoprotein B-lipoprotein-associated phospholipase A(2) (apoB-Lp-PLA(2)) levels. This suggests a potential dietary intervention for cardiovascular risk markers.

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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Area of Science:

Cardiovascular Disease Research
Lipid Metabolism Studies
Nutritional Science

Background:

Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) is a key cardiovascular risk marker.
Lp-PLA(2) is primarily associated with apolipoprotein B (apoB)-containing lipoproteins.
Dietary sodium intake may influence apoB-lipoprotein levels.

Purpose of the Study:

To investigate the effect of a sodium challenge on plasma Lp-PLA(2) mass.
To determine if a sodium challenge alters apoB-lipoprotein particles carrying Lp-PLA(2) (apoB-Lp-PLA(2)).

Main Methods:

A 3-day oral sodium chloride challenge (9 g/day) was administered to 76 participants (45 women, 31 men).
Plasma Lp-PLA(2) mass was measured using turbidimetric immunoassay.
Levels of apoB-Lp-PLA(2) were assessed using a newly developed enzyme-linked immunosorbent assay.

Main Results:

Sodium loading significantly increased urinary sodium excretion.
Plasma Lp-PLA(2) mass and apoB-Lp-PLA(2) levels showed significant decreases post-challenge.
Associated reductions were observed in total cholesterol, non-HDL cholesterol, LDL cholesterol, apoB, and total cholesterol/HDL cholesterol ratio.

Conclusions:

Short-term oral sodium intake effectively reduces both plasma Lp-PLA(2) mass and apoB-Lp-PLA(2) levels.
These findings highlight a potential dietary strategy for managing cardiovascular risk markers.