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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

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Related Experiment Video

Updated: May 18, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

NG6: Integrated next generation sequencing storage and processing environment.

Jérôme Mariette¹, Frédéric Escudié, Nicolas Allias

¹Plate-forme bio-informatique Genotoul, INRA, Biométrie et Intelligence Artificielle, BP 52627, 31326, Castanet-Tolosan Cedex, France. Jerome.Mariette@toulouse.inra.fr

|September 11, 2012

Summary

This summary is machine-generated.

NG6 is a user-friendly information system for managing next-generation sequencing data. It offers customizable workflows and a secure web interface for processing, storing, and downloading sequencing reads and analysis results.

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Related Experiment Videos

Last Updated: May 18, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

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Published on: April 11, 2016

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Area of Science:

Bioinformatics
Genomics
Computational Biology

Background:

Next-generation sequencing (NGS) platforms are increasingly common in research labs.
Smaller-scale sequencers will further expand NGS accessibility.
There is a need for manageable systems to store and process NGS data.

Purpose of the Study:

To describe a user-friendly information system for managing large sets of sequencing data.
To provide a solution for laboratories with limited bioinformatics support.
To facilitate data processing and analysis for various sequencing applications.

Main Methods:

Development of an integrated information system (NG6).
Inclusion of a workflow environment with pre-built pipelines for various input formats (sff, fasta, fastq, qseq) and sequencers (Roche 454, Illumina HiSeq).
Integration of a secure web interface for data access and analysis result browsing.
Utilized Ergatis for workflow management and supported local or cluster execution (Sun Grid Engine).

Main Results:

A comprehensive system (NG6) for managing high-throughput sequencing data.
User-friendly interface for processing, storing, and downloading raw and processed sequencing data.
Customizable workflows supporting diverse analyses like quality control, assembly, alignment, and diversity studies.
Secure web access to results and analysis statistics.

Conclusions:

NG6 is a complete information system tailored for sequencing platforms.
It addresses the need for user-friendly processing, storage, and download of high-throughput sequencing data.
The system enhances the capabilities of laboratories utilizing next-generation sequencing.