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Published on: October 12, 2015
Fluorometric enzymatic autoindicating biosensor for H2O2 determination based on modified catalase
Estefania Ortega1, Susana de Marcos, Javier Galbán
1Bioanalytical sensors group (GBA), Analytical Chemistry Department, Faculty of Sciences, Aragon Institute of Nanosciences of Aragon (INA), University of Zaragoza, Pedro Cerbuna 12, Zaragoza 50009, Spain.
Biosensors & Bioelectronics
|September 11, 2012
Summary
This study introduces a novel reversible optical biosensor for continuous hydrogen peroxide (H2O2) monitoring. The developed sensor, utilizing catalase and a ruthenium fluorophore, offers reliable H2O2 determination for at least one month.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biosensor Technology
Background:
- Continuous monitoring of hydrogen peroxide (H2O2) is crucial in various scientific and medical applications.
- Existing biosensors often lack reversibility, limiting their use for prolonged measurements.
- Development of robust and reusable biosensors is essential for efficient analytical processes.
Purpose of the Study:
- To develop a reversible optical biosensor for continuous hydrogen peroxide (H2O2) determination.
- To optimize a fluorescent-enzymatic system for H2O2 detection using catalase and a ruthenium fluorophore.
- To create a stable immobilized sensor for long-term H2O2 monitoring and develop a predictive mathematical model.
Main Methods:
- Optimization of a catalase-ruthenium fluorophore (Cat-Ru) system for batch H2O2 measurements.
- Immobilization of the Cat-Ru system in a polyacrylamide film to create an optical sensor.
- Characterization of sensor performance, including linear response range and operational stability.
- Development of a mathematical model to predict sensor behavior and experimental parameter effects.
- Evaluation of an alternative sensor using a commercial oxygen fluorescence sensor linked to catalase.
Main Results:
- The fluorescent-enzymatic system demonstrated a linear response range for H2O2 from 1x10(-4) to at least 1x10(-3) M.
- The immobilized optical sensor enabled H2O2 determination in a similar concentration range and maintained functionality for over one month.
- The mathematical model accurately predicted sensor performance and demonstrated minimal dependence on initial oxygen concentration.
- An alternative sensor configuration yielded comparable analytical performance.
Conclusions:
- A reversible optical biosensor for H2O2 determination has been successfully developed and validated.
- The immobilized sensor offers a stable and reusable platform for continuous H2O2 monitoring.
- The developed mathematical model provides valuable insights into sensor design and performance optimization.
