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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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Related Experiment Video

Updated: May 18, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Long noncoding RNAs with snoRNA ends.

Qing-Fei Yin1, Li Yang, Yang Zhang

  • 1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Molecular Cell
|September 11, 2012
PubMed
Summary
This summary is machine-generated.

Researchers discovered snoRNA-processed long noncoding RNAs (sno-lncRNAs). These molecules are implicated in Prader-Willi Syndrome (PWS) pathogenesis by altering gene splicing patterns.

Related Experiment Videos

Last Updated: May 18, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in cellular processes.
  • The processing and function of many lncRNAs remain incompletely understood.
  • Intron-derived lncRNAs represent a significant, yet understudied, class of noncoding transcripts.

Purpose of the Study:

  • To identify and characterize novel classes of lncRNAs.
  • To investigate the biogenesis and cellular localization of these newly discovered RNAs.
  • To explore the potential role of these lncRNAs in human diseases, specifically Prader-Willi Syndrome (PWS).

Main Methods:

  • RNA sequencing and analysis to identify novel RNA species.
  • Biochemical assays to determine RNA processing and localization.
  • Molecular techniques to study RNA-protein interactions and functional consequences.
  • Analysis of patient-derived samples with genetic deletions in specific chromosomal regions.

Main Results:

  • Discovery of snoRNA-processed long noncoding RNAs (sno-lncRNAs), which are intron-derived and processed by the snoRNA machinery.
  • These sno-lncRNAs accumulate in the nucleus, lacking 5' caps and 3' poly(A) tails.
  • A specific class of sno-lncRNAs encoded in the 15q11-q13 region is deleted in Prader-Willi Syndrome.
  • These PWS-associated sno-lncRNAs associate with splicing regulators and alter splicing patterns.

Conclusions:

  • A novel class of nuclear-enriched, intron-derived lncRNAs processed by snoRNA machinery has been identified.
  • These sno-lncRNAs are widely expressed and play a role in regulating gene splicing.
  • The findings implicate sno-lncRNAs in the molecular pathogenesis of Prader-Willi Syndrome, highlighting a new avenue for understanding this complex genetic disorder.