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Updated: May 18, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Recent developments in liquid chromatography-mass spectrometry and related techniques
Michal Holčapek1, Robert Jirásko, Miroslav Lísa
1Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic. Michal.Holcapek@upce.cz
This review covers recent advancements in liquid chromatography-mass spectrometry (LC-MS) and related techniques. It provides an overview of LC-MS systems, discussing their pros and cons for various analytical applications.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Analysis
- Clinical Analysis
Background:
- Liquid chromatography-mass spectrometry (LC-MS) has seen significant growth due to its wide applicability.
- Characterizing complex organic, bioorganic, and organometallic mixtures is crucial across scientific disciplines.
- The variety of LC-MS systems can be overwhelming for users.
Purpose of the Study:
- To review the state-of-the-art in LC-MS and related techniques, focusing on the last decade.
- To provide an overview and independent assessment of mass spectrometers for LC-MS and MALDI-MS configurations.
- To discuss current trends and emerging approaches in mass spectrometry relevant to LC-MS.
Main Methods:
- Review of recent developments in LC-MS and related mass spectrometry techniques.
- Compilation of an overview of mass spectrometers from major manufacturers for LC-MS and MALDI-MS.
- Independent assessment of the advantages and limitations of various LC-MS systems.
Main Results:
- Detailed discussion of recent advancements in LC-MS technology.
- Comparative analysis of different mass spectrometers and their suitability for LC-MS and MALDI-MS.
- Insights into current trends including fast LC-MS, ion mobility, and quantitation strategies.
Conclusions:
- LC-MS is a powerful tool for analyzing complex mixtures in diverse fields.
- Understanding system variations and trends is essential for effective LC-MS application.
- Emerging mass spectrometric techniques offer complementary capabilities to LC-MS.
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