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A basic study on hypericin-PDT in vitro.

Nobue Nakajima1, Norimichi Kawashima

  • 1Toin University of Yokohama, Biomedical Engineering Center, 1614 Kuroganecho, Aobaku, Yokohama 225-8502, Japan. n.nobue@cc.toin.ac.jp

Photodiagnosis and Photodynamic Therapy
|September 11, 2012
PubMed
Summary

Photodynamic therapy (PDT) using hypericin effectively induces apoptosis in U937 cancer cells. Optimal results were achieved with specific LED lamps and a 3-hour incubation period post-irradiation, leading to near-complete cell death.

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Area of Science:

  • Biomedical Science
  • Photochemistry
  • Cell Biology

Background:

  • Photodynamic therapy (PDT) is a promising cancer treatment modality.
  • Hypericin serves as a photosensitizer in PDT.
  • Understanding PDT effects on cancer cell lines like U937 is crucial.

Purpose of the Study:

  • To investigate the efficacy of hypericin-PDT on U937 cells.
  • To evaluate the impact of different light sources on PDT outcomes.
  • To determine optimal incubation times and light parameters for cancer cell apoptosis.

Main Methods:

  • U937 cells were treated with hypericin and exposed to various light sources (Na-Li lamp, LED lamps).
  • Cell viability and intracellular ATP levels were assessed post-irradiation.

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  • Apoptosis rates were quantified following different incubation periods.

    • Main Results:

    • Hypericin penetration into U937 cells required 1 hour, with effects observed after 3 hours post-irradiation.
    • Na-Li lamp irradiation (5 min) resulted in 55% cell viability decrease immediately, approaching 0% after 5 hours.
    • LED lamps (599 nm, 595 nm) achieved near-complete cell death after 5-hour incubation following 15-minute irradiation.

    Conclusions:

    • Hypericin-PDT is effective in inducing apoptosis in U937 cells.
    • A post-irradiation incubation period exceeding 3 hours is necessary for maximal cell death.
    • Low-intensity LED lamps, combined with appropriate hypericin concentration and incubation, yield superior apoptosis rates in U937 cells.