Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
Steps in Outbreak Investigation01:18

Steps in Outbreak Investigation

In the ever-evolving field of public health, statistical analysis serves as a cornerstone for understanding and managing disease outbreaks. By leveraging various statistical tools, health professionals can predict potential outbreaks, analyze ongoing situations, and devise effective responses to mitigate impact. For that to happen, there are a few possible stages of the analysis:
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Leaf Extracts of Cornus sanguinea L., C. sericea L., and C. alba L. on Digestive Enzymes and Blood Coagulation In Vitro.

Plant foods for human nutrition (Dordrecht, Netherlands)·2026
Same author

Integrating machine learning and multi-criteria decision analysis for health risk management in water distribution networks.

Scientific reports·2026
Same author

Variations of the Virome in Raw and Treated Water: A One-Year Follow-Up at Six Different Drinking Water Treatment Plants.

Environmental microbiology reports·2025
Same author

Development and validation of a new method for predicting spatial and temporal concentration dynamics of active pharmaceutical ingredients in a lake receiving wastewater effluents.

Water research·2025
Same author

Inhibitory potential of sea buckthorn leaves extracts and its component casuarictin on pancreatic enzymes and LPS induced TNF-alpha synthesis in human whole blood.

Natural product research·2025
Same author

Structure, function and catalytic mechanism of the carrageenan-sulfatases from the marine bacterium Zobellia galactanivorans Dsij<sup>T</sup>.

Carbohydrate polymers·2025

Related Experiment Video

Updated: May 18, 2026

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

Estimation of pathogen concentrations in a drinking water source using hydrodynamic modelling and microbial source

Ekaterina Sokolova1, Johan Aström, Thomas J R Pettersson

  • 1Water Environment Technology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden. ekaterina.sokolova@chalmers.se

Journal of Water and Health
|September 11, 2012
PubMed
Summary

Estimating pathogen levels in drinking water sources is crucial for preventing waterborne diseases. This study introduces a new method combining microbial source tracking and modeling to predict pathogen concentrations and assess risks.

More Related Videos

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

Related Experiment Videos

Last Updated: May 18, 2026

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
12:14

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

Area of Science:

  • Environmental microbiology
  • Water quality assessment
  • Public health

Background:

  • Faecal contamination of drinking water sources poses a significant risk for waterborne disease outbreaks.
  • Accurate estimation of pathogen concentrations in raw water is essential for risk assessment.
  • Current monitoring methods have limitations in providing comprehensive data.

Purpose of the Study:

  • To develop and present a novel approach for estimating pathogen concentrations in drinking water sources.
  • To utilize microbial source tracking data and fate and transport modeling for risk assessment.
  • To provide data for quantitative microbial risk assessment (QMRA) and risk management.

Main Methods:

  • Estimated pathogen concentrations (norovirus, Cryptosporidium, Escherichia coli O157/H7) in faecal sources using indicator organisms (E. coli, Bacteroidales).
  • Simulated pathogen fate and transport in Lake Rådasjön using a 3D hydrodynamic and microbiological model.
  • Assessed the contribution of different contamination sources to pathogen levels at the water intake.

Main Results:

  • Successfully estimated pathogen concentrations in faecal sources under endemic and epidemic conditions.
  • Modeled the complex movement and behavior of pathogens within the lake ecosystem.
  • Quantified the impact of various contamination sources on raw water quality at the intake.

Conclusions:

  • The novel approach effectively estimates pathogen concentrations and source contributions in drinking water.
  • This method overcomes limitations of traditional monitoring, enhancing QMRA and risk management strategies.
  • Improved understanding of pathogen dynamics in surface water sources aids in safeguarding drinking water supplies.