Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simultaneous ANAMMOX and denitrification (SAD) process in batch tests.

Yu Ming Zhong1, Xiao Shan Jia

  • 1School of Environmental Science and Engineering, Sun Yat-sen University, 510275 Guangzhou, China. unicornyy@gmail.com

World Journal of Microbiology & Biotechnology
|September 11, 2012
PubMed
Summary

Simultaneous anaerobic ammonia oxidation and nitrate reduction (SAD) processes were clarified, showing nitrite consumption and ammonia oxidation. Microbial cooperation and nutrient ratios influence this process for potential wastewater treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Diversity, biofilm formation and antimicrobial susceptibility of aerobic heterotrophic bacteria isolated from cooling towers.

World journal of microbiology & biotechnology·2026
Same journal

From gene to enzyme: Microbial engineering of bovine chymosin for efficient cheese coagulation.

World journal of microbiology & biotechnology·2026
Same journal

Metabolic engineering strategies of Corynebacterium glutamicum for sulfur-containing amino acid production: current progress and future directions.

World journal of microbiology & biotechnology·2026
Same journal

Recombinant expression of natural Arabidopsis PRLIP1 variants reveals temperature-dependent differences in fluorescence and functional protein recovery in Escherichia coli.

World journal of microbiology & biotechnology·2026
Same journal

Phenotypic and genomic insights into Stenotrophomonas sepilia SMBL8: metabolic versatility, antibiotic resistance and pathogenic potential.

World journal of microbiology & biotechnology·2026
Same journal

Horizontal gene transfer in Saccharomyces cerevisiae and other Saccharomycotina yeasts: a review.

World journal of microbiology & biotechnology·2026

Area of Science:

  • Environmental microbiology
  • Biogeochemical cycles

Background:

  • Simultaneous anaerobic ammonia oxidation and nitrate reduction (SAD) is a crucial process in nitrogen cycling.
  • Understanding the factors influencing SAD is vital for optimizing nitrogen removal in wastewater treatment.

Purpose of the Study:

  • To elucidate the occurrence and dynamics of SAD processes in enrichment cultures with glucose.
  • To investigate the impact of autotrophic and mixotrophic conditions on nitrate conversion and by-product formation.
  • To assess the effects of methanol and penicillin G on SAD activity.

Main Methods:

  • Batch tests were employed to study SAD processes.
  • Nitrate conversion and by-product formation were monitored.
  • Scanning electron microscopy (SEM) and denaturing gradient gel electrophoresis (DGGE) were used for microbial analysis.

Related Experiment Videos

Main Results:

  • Nitrite was fully consumed, co-occurring with ammonia oxidation under mixotrophic conditions.
  • Methanol inhibited ANAMMOX activity at low concentrations (1 mM), while penicillin G had minimal impact.
  • SEM and DGGE confirmed the involvement of ANAMMOX bacteria and co-existing bacteria in the SAD process.

Conclusions:

  • The ratio of nitrogen to organic carbon and microbial interactions significantly affect SAD process dynamics.
  • The SAD process shows potential for ammonia and nitrate removal from wastewater containing organic matter.