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Redesign of the coenzyme specificity of a dehydrogenase by protein engineering
N S Scrutton1, A Berry, R N Perham
1Department of Biochemistry, University of Cambridge, UK.
Nature
|January 4, 1990
Abstract:
Directed mutagenesis and molecular modelling have been used to identify a set of amino-acid side chains in glutathione reductase that confer specificity for the coenzyme NADP+. Systematic replacement of these amino acids, all of which occur in a 'fingerprint' structural motif in the NADP+-binding domain, leaves the substrate specificity unchanged but converts the enzyme into one displaying a marked preference for the coenzyme NAD+.