Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence-based docking of the urease activation complex.

Rodrigo Ligabue-Braun1, Rafael Real-Guerra, Célia Regina Carlini

  • 1Graduate Program in Cellular and Molecular Biology, Center of Biotechnology, Universidade Federal do Rio Grande do Sul-UFRGS, Porto Alegre, RS, Brazil.

Journal of Biomolecular Structure & Dynamics
|September 12, 2012
PubMed
Summary

Researchers developed an atomic-level model of the Klebsiella aerogenes urease activation complex (UreABC-UreDFG)₃. This provides crucial insights into how urease enzymes are activated and function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular 70 kDa heat shock protein in blood plasma binds insulin and modulates glycaemic control in vivo.

Cell stress & chaperones·2026
Same author

Jack bean urease impairs oogenesis in the Chagas disease vector Rhodnius prolixus.

International journal of biological macromolecules·2026
Same author

Characterization of Novel Angiotensin-Converting Enzyme Inhibitory Peptides.

ACS medicinal chemistry letters·2025
Same author

eRMSF: A Python Package for Ensemble-Based RMSF Analysis of Biomolecular Systems.

Journal of chemical information and modeling·2025
Same author

Targeting KRAS<sup>G13C</sup> with cyclic linker based inhibitors to explore warhead orientation.

Scientific reports·2025
Same author

Properties and Pharmacology of Scorpion Toxins and Their Biotechnological Potential in Agriculture and Medicine.

Toxins·2025

Area of Science:

  • Biochemistry
  • Structural Biology
  • Microbiology

Background:

  • Urease enzymes require accessory proteins for activation and function.
  • In Klebsiella aerogenes, urease activation involves sequential complexation of UreD, UreF, UreG, and UreE with UreABC.
  • Previous structural studies of this complex were limited to low resolution.

Purpose of the Study:

  • To generate an atomic-level model of the (UreABC-UreDFG)₃ complex from Klebsiella aerogenes.
  • To elucidate the mechanism of urease activation at a molecular level.

Main Methods:

  • Comparative modeling and sequential macromolecular docking were employed.
  • The atomic model was validated using small-angle X-ray scattering (SAXS) profiles.
  • Experimental data from cross-linking, mutagenesis, and pull-down assays were used for validation.

Related Experiment Videos

Main Results:

  • An atomic-level model of the (UreABC-UreDFG)₃ complex was successfully generated.
  • Normal mode analyses revealed significant flexibility in the UreD-UreF dimer and the overall complex.
  • The flexibility explains the previously observed diffuse binding of UreD to the apoenzyme.

Conclusions:

  • This study presents the first atomic-level model of the urease activation complex.
  • The model is essential for understanding the intricate process of urease activation.
  • The findings provide a foundation for future research into urease function and regulation.