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Related Experiment Videos

Decrease of polymorphonuclear leukocyte membrane fluidity in uremic patients on hemodialysis.

M Masuda1, Y Komiyama, T Murakami

  • 1Department of Clinicolaboratory Medicine, Kansai Medical University, Osaka, Japan.

Nephron
|January 1, 1990
PubMed
Summary
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Membrane fluidity in polymorphonuclear leukocytes (PMN) is reduced in uremic patients. Hemodialysis normalizes PMN fluidity, suggesting removal of serum factors responsible for this change.

Area of Science:

  • Nephrology
  • Immunology
  • Biochemistry

Background:

  • Uremic patients undergoing hemodialysis often exhibit an increased susceptibility to infections.
  • Polymorphonuclear leukocytes (PMN) play a crucial role in the immune response.
  • Altered cellular membrane properties may contribute to impaired immune function in uremia.

Purpose of the Study:

  • To investigate the membrane fluidity of PMN in patients with uremia undergoing hemodialysis.
  • To determine if hemodialysis affects PMN membrane fluidity.
  • To identify serum factors influencing PMN membrane fluidity in uremia.

Main Methods:

  • Measurement of PMN membrane fluidity using the excimer-forming lipid technique with pyrenedecanoic acid.
  • Flow cytometry was employed for quantitative analysis.

Related Experiment Videos

  • Serum fractionation via Sephadex G-25 column chromatography was performed.

    • Main Results:

    • PMN membrane fluidity was significantly reduced in uremic patients before hemodialysis.
    • Hemodialysis led to a progressive normalization of PMN membrane fluidity.
    • Cross-incubation studies confirmed the presence of serum factors affecting fluidity, which are removable by hemodialysis.
    • Fractionation identified low-molecular-weight serum components as responsible for decreased fluidity.

    Conclusions:

    • Uremia is associated with significantly reduced PMN membrane fluidity.
    • Hemodialysis effectively improves PMN membrane fluidity by removing specific serum factors.
    • Altered PMN membrane fluidity may be a contributing factor to the increased infection risk in uremic patients.