Related Experiment Video
Updated: May 18, 2026
Establishment of an Embryo Implantation Model In Vitro
Published on: June 21, 2024
Development and characterization of an endometrial tissue culture model for study of early implantation events
Tian-Min Ye1, Ronald T K Pang, Carmen O N Leung
1Department of Obstetrics and Gynecology, University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China.
Objective:
To improve and characterize an endometrial tissue culture model.
Design:
Experimental study of the characteristics of mouse endometrial tissue cultured on amniotic membrane matrix.
Setting:
University research laboratory.
Animal(S):
Sexually mature female ICR mice.
Intervention(S):
Histologic examination of the cultured endometrial tissues. The attachment rates of the cultured tissues to implantation blastocysts under various conditions were determined.
Main Outcome Measure(S):
Morphometric analysis of the cultured tissues. Blastocyst attachment rate and expression of decidualization markers cylcooxygenase-2, connexin 43, and peroxisome proliferator-activated receptor δ.
Result(S):
Endometrial tissues could be grown on amniotic membrane matrix for 3 days with morphometric parameters similar to those in the in vivo pseudopregnant control. The cultured tissues responded to the surrounding steroid environment. Morphometric assessment indicated that medium containing 63.5 nmol/L P and 0.9 nmol/L E(2) provided the best support. The condition allowed attachment of approximately 60% of the cocultured blastocysts. A small percentage of the attached blastocyst started to penetrate the luminal epithelium within 28 hours. The attachment rate was significantly reduced with prior treatment of the cultured endometrium with anti-leukemia inhibitory factor antibody. The attached blastocyst induced decidualization around the attachment site.
Conclusion(S):
The model is useful for the study on implantation in the mouse.
