Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumor-associated macrophages display differential protein cargo sorting in extracellular vesicles associated with poor survival in ovarian cancer.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies.

Advanced healthcare materials·2026
Same author

Extracellular vesicles from ovarian cancer tumor spheroids harbor disease-related and survival-associated proteins.

Extracellular vesicles and circulating nucleic acids·2026
Same author

Adverse impact of acute Toxoplasma gondii infection on human spermatozoa.

The FEBS journal·2025
Same author

Bovine PMN responses to extracellular vesicles released by <i>Besnoitia besnoiti</i> tachyzoites and <i>B. besnoiti</i>-infected host cells.

Frontiers in immunology·2025
Same author

Isolation of native EVs from primary biofluids-Free-flow electrophoresis as a novel approach to purify ascites-derived EVs.

Journal of extracellular biology·2024
Same journal

Successful treatment of rare non-dermatophyte onychomycosis caused by Chaetomium spp. with oral fluconazole and topical terbinafine combination - A case report from Indonesia and literature review.

International journal of medical microbiology : IJMM·2026
Same journal

Toxin gene truncation via IS1132 in non-toxigenic toxin gene-bearing (NTTB) Corynebacterium diphtheriae.

International journal of medical microbiology : IJMM·2026
Same journal

Gain-of-function mutation in the sensor kinase CpxS modulates the antimicrobial resistance and virulence of Pseudomonas aeruginosa.

International journal of medical microbiology : IJMM·2026
Same journal

Mycobacterium tuberculosis identification and antibiotic resistance gene profiling using nanopore sequencing: A new approach for tailoring more effective treatments for patients.

International journal of medical microbiology : IJMM·2026
Same journal

Epidemiology and population structure of Acinetobacter baumannii outbreak in a COVID-19 concentration center.

International journal of medical microbiology : IJMM·2026
Same journal

Herpes simplex virus 1 UL2 protein inhibits RIG-I-like receptor pathway-induced IFN-β activity by disrupting IRF3 activation.

International journal of medical microbiology : IJMM·2026
See all related articles

Related Experiment Video

Updated: May 18, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

mRNA splicing in trypanosomes.

Christian Preußer1, Nicolas Jaé, Albrecht Bindereif

  • 1Institute of Biochemistry, Justus Liebig University of Giessen, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany.

International Journal of Medical Microbiology : IJMM
|September 12, 2012
PubMed
Summary
This summary is machine-generated.

Trans-splicing in trypanosomatids generates mature mRNAs by attaching a spliced leader (SL) RNA sequence. Alternative trans-splicing expands mRNA and protein diversity in these parasitic organisms.

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Related Experiment Videos

Last Updated: May 18, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Parasitology

Background:

  • Trypanosomatids are parasitic unicellular organisms causing fatal human and livestock diseases.
  • They exhibit unique molecular features, including polycistronic transcription of protein-coding genes.

Purpose of the Study:

  • To provide an overview of the current research on trans-splicing in trypanosomatids.
  • To highlight the role of trans-splicing in generating mRNA diversity.

Main Methods:

  • Review of recent genome-wide studies.
  • Analysis of biochemical and molecular biology data on trans-splicing.

Main Results:

  • Trans-splicing, coupled with polyadenylation, processes long primary transcripts into mature mRNAs.
  • A 39-nucleotide spliced leader (SL) RNA sequence is attached to protein-coding genes via trans-splicing.
  • Alternative trans-splicing significantly increases mRNA and protein diversity.

Conclusions:

  • Trans-splicing is a crucial mechanism for gene expression in trypanosomatids.
  • Understanding trans-splicing is key to comprehending the biology of these important parasites.