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Updated: May 18, 2026
Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor
Published on: May 2, 2022
The clinical significance of reduced programmed cell death 5 expression in human gastrointestinal stromal tumors
Fei Gao1, Lingling Ding, Miaoqing Zhao
1Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Shandong University Qilu Hospital, Jinan, Shandong, PR China. gaofeijn@yahoo.com.cn
Abstract:
Programmed cell death 5 (PDCD5) is a novel apoptosis-related gene with potent antitumor effects which can interact with the histone acetyltransferase Tip60 and induce DNA damage-induced apoptosis. Reduced PDCD5 expression has been found in a few types of human tumors and is also associated with the progression and prognosis of the tumors. However, the expression status and clinical significance of PDCD5 in gastrointestinal stromal tumors has not yet been analyzed. In our study, we examined PDCD5 expression in 63 tumor samples of gastrointestinal stroma at both mRNA and protein levels by RT-PCR, western blotting and immunohistochemistry. We found that 57% (16/28) of the tumor samples had a decreased PDCD5 expression at the mRNA level and 53% (35/66) of the samples were found to have decreased PDCD5 expression at the protein level, whereas PDCD5 was highly expressed in all adjacent normal gastrointestinal tissues at the mRNA or protein level. Moreover, decreased PDCD5 expression was significantly associated with clinicopathological characteristics including tumor size and mitosis. Our results suggest that PDCD5 expression plays a significant role in the malignant progression of human gastrointestinal stromal tumors and may be a key inhibitory factor.
Insights
Programmed cell death 5 (PDCD5) is often reduced in gastrointestinal stromal tumors, correlating with tumor progression. This suggests PDCD5 may act as a key tumor suppressor in these cancers.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Programmed cell death 5 (PDCD5) is an apoptosis-related gene with known antitumor properties.
- Reduced PDCD5 expression is linked to tumor progression and poor prognosis in some cancers.
- The role of PDCD5 in gastrointestinal stromal tumors (GIST) remains uninvestigated.
Purpose of the Study:
- To investigate the expression status of PDCD5 in GIST.
- To determine the clinical significance of PDCD5 expression in GIST.
Main Methods:
- Examined PDCD5 mRNA and protein levels in 63 GIST samples.
- Utilized RT-PCR, western blotting, and immunohistochemistry.
- Compared tumor tissue expression with adjacent normal gastrointestinal tissues.
Main Results:
- Decreased PDCD5 mRNA expression was observed in 57% of GIST samples.
- Reduced PDCD5 protein levels were found in 53% of GIST samples.
- PDCD5 was highly expressed in normal gastrointestinal tissues.
- Decreased PDCD5 expression correlated significantly with larger tumor size and higher mitosis rates.
Conclusions:
- PDCD5 expression is significantly downregulated in GIST.
- Reduced PDCD5 is associated with key clinicopathological features of GIST.
- PDCD5 may function as a crucial tumor suppressor in the malignant progression of GIST.
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