Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endocannabinoid cerebrospinal fluid levels in migraine and its relation to symptoms of depression.

Cephalalgia : an international journal of headache·2026
Same author

Supporting-like cells constitute an alternative steroidogenic lineage conserved in amniotes.

bioRxiv : the preprint server for biology·2026
Same author

Cannabis consumption is associated with altered steroid metabolism in young men.

Communications medicine·2026
Same author

Heterologous internal calibration for multiplexed internal quantification in targeted LC-MS/MS bioanalysis.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Characterization of residual kidney function in chronic hemodialysis patients using plasma metabolomics.

Scientific reports·2026
Same author

Simultaneous analysis of various anticancer drugs by supercritical fluid chromatography-mass spectrometry. Part II: Method validation and comparison with liquid chromatography.

Journal of pharmaceutical and biomedical analysis·2026

Related Experiment Video

Updated: May 18, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Microextraction techniques combined with capillary electrophoresis in bioanalysis.

Isabelle Kohler1, Julie Schappler, Serge Rudaz

  • 1School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Bd d'Yvoy 20, 1211 Geneva 4, Switzerland.

Analytical and Bioanalytical Chemistry
|September 12, 2012
PubMed
Summary

Environmentally sustainable microextraction techniques (MEs) combined with capillary electrophoresis (CE) offer greener bioanalysis. This review covers liquid and solid-phase MEs, highlighting their application in analyzing small biological samples with reduced solvent use.

More Related Videos

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
11:39

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Published on: June 9, 2019

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Related Experiment Videos

Last Updated: May 18, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
11:39

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Published on: June 9, 2019

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Area of Science:

  • Analytical Chemistry
  • Environmental Chemistry

Background:

  • Growing demand for sustainable analytical methods to reduce toxic organic solvent use.
  • Microextraction techniques (MEs) minimize solvent consumption and sample volume, ideal for bioanalysis.
  • Capillary electrophoresis (CE) is a solvent-free separation technique, enhancing environmental compatibility.

Purpose of the Study:

  • To review the application of microextraction techniques (MEs) coupled with capillary electrophoresis (CE) in bioanalysis.
  • To provide a comprehensive overview of environmentally sustainable sample preparation methods.
  • To discuss both liquid-phase and solid-phase MEs in combination with CE.

Main Methods:

  • Review of existing literature on MEs and CE in bioanalysis.
  • Categorization of MEs into liquid-based and solid-based techniques.
  • Inclusion of practical and theoretical descriptions of each ME.

Main Results:

  • MEs combined with CE represent a powerful, environmentally friendly analytical tool for bioanalysis.
  • Numerous applications reported for low-molecular-weight compounds and peptide analysis.
  • Demonstrated advantages include reduced sample volume, analysis time, and operating costs.

Conclusions:

  • The combination of MEs and CE is a highly attractive, sustainable approach for bioanalytical sample preparation.
  • This review highlights the versatility and effectiveness of these techniques for complex biological samples.
  • Future applications are expected to expand due to the environmental and economic benefits.