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The Equilibrium Binding Constant and Binding Strength

The Equilibrium Binding Constant and Binding Strength

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Related Experiment Video

Updated: May 18, 2026

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

Published on: March 14, 2016

MS-based ligand binding assays with speed, sensitivity, and specificity.

Michael J Roth¹, Jaekuk Kim, Erica M Maresh

¹Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9185, USA.

|September 12, 2012

Summary

This summary is machine-generated.

This study introduces a novel matrix deposition technique for immunoassays, enabling sensitive and specific quantification of proteins. This advancement allows for precise analysis of antigens at picomolar levels using mass spectrometry.

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Last Updated: May 18, 2026

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Area of Science:

Biochemistry
Analytical Chemistry
Biophysics

Background:

Immunoassays are essential laboratory tools due to their simplicity, speed, and sensitivity.
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) offers high sensitivity and specificity for molecular analysis.

Purpose of the Study:

To develop a highly sensitive and specific immunoassay method by combining self-assembled monolayers with MALDI-TOF MS.
To achieve quantitative analysis of antigens with picomolar detection limits.

Main Methods:

Utilized self-assembled monolayer (SAM) technology for surface preparation.
Developed a novel matrix deposition and crystallization technique for MALDI-TOF MS.
Performed quantitative analysis of monolayer-bound antigens.

Main Results:

Achieved high-fidelity surface preparations enabling quantitative analysis of bound antigens.
Demonstrated picomolar detection limits for antigens.
Established calibration curves for intact proteins over a broad concentration range.
Showcased simultaneous label-free quantitation of ligand-bound protein complexes, improving specificity.

Conclusions:

The combined SAM-based immunoassay and MALDI-TOF MS approach offers a powerful platform for sensitive and specific protein quantification.
The novel matrix deposition technique is crucial for achieving high-fidelity results and low detection limits.
This method enhances the specificity of mass spectrometry-immunoassays.