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Related Experiment Video

Updated: May 18, 2026

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Published on: December 26, 2014

Lipocalin-2 ameliorates granulocyte functionality.

Andrea Schroll¹, Kathrin Eller, Clemens Feistritzer

¹Department of Internal Medicine, Clinical Immunology and Infectious Diseases, Medical University Innsbruck, Innsbruck, Austria.

European Journal of Immunology

|September 12, 2012

Summary

This summary is machine-generated.

Neutrophil gelatinase-associated lipocalin (Lcn2) is a key regulator of neutrophil attraction to infection sites. This study reveals Lcn2

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Rapid Magnetic-microbead Method for Efficient Purification of Low-density Neutrophils

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Published on: November 11, 2025

Area of Science:

Immunology
Cell Biology
Biochemistry

Background:

Neutrophil recruitment is crucial for innate immunity.
Neutrophil gelatinase-associated lipocalin (Lcn2) is an antimicrobial protein produced by neutrophils.

Purpose of the Study:

To investigate the role of Lcn2 in neutrophil migration and function.
To elucidate the mechanism by which Lcn2 regulates neutrophil chemotaxis.

Main Methods:

Recombinant Lcn2 was added to human and murine neutrophils.
Lcn2 knockout (KO) and wild-type (WT) mice were used.
Neutrophil migration, mobilization, and adhesion assays were performed.
Salmonella typhimurium infection model was utilized.

Main Results:

Recombinant Lcn2 significantly stimulated neutrophil migration, independent of IL-8.
Lcn2 affects Erk1/2 signaling pathways.
Lcn2 injection mobilized neutrophils in vivo.
Lcn2 KO mice showed reduced neutrophil chemotaxis and impaired adhesion.
Lcn2 KO mice exhibited reduced neutrophil accumulation at infection sites.

Conclusions:

Lcn2 acts as a novel paracrine chemoattractant for neutrophils.
Lcn2 is essential for efficient neutrophil function during inflammation.
Lcn2 plays a critical role in the innate immune response to infection.