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Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

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Updated: May 18, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

MultiBac complexomics.

Simon Trowitzsch1, Dieter Palmberger, Daniel Fitzgerald

  • 1European Molecular Biology Laboratory and Unit of Virus Host Cell Interactions, CNRS-EMBL-UJF UMR 5322, 6 rue Jules Horowitz, F-38042 Grenoble Cedex 9, France.

Expert Review of Proteomics
|September 13, 2012
PubMed
Summary
This summary is machine-generated.

MultiBac, a baculovirus expression technology, enables simultaneous recombinant protein complex production in insect cells. This open-access platform advances research in health, disease, and glycosylated protein development.

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Last Updated: May 18, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
09:29

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Published on: May 15, 2019

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Structural Biology

Background:

  • Recombinant production of multiprotein complexes is crucial for understanding biological processes in health and disease.
  • Existing methods face challenges in efficiently producing complex protein assemblies.
  • There is a growing need for advanced expression systems in academic and pharmaceutical research.

Purpose of the Study:

  • To describe the MultiBac system, a state-of-the-art eukaryotic expression technology.
  • To detail the performance and accessibility of the MultiBac platform at EMBL Grenoble.
  • To present SweetBac, a novel modification for producing glycosylated protein targets.

Main Methods:

  • Utilizing an engineered baculovirus for expression in insect cells.
  • Implementing MultiBac for simultaneous expression of multiple proteins within a single cell.
  • Employing the SweetBac system for customized production of glycosylated proteins.

Main Results:

  • MultiBac facilitates the robust and flexible production of multiprotein complexes.
  • The system has been successfully applied to elucidate the function of essential multiprotein complexes.
  • The open-access platform at EMBL Grenoble enhances accessibility for the scientific community.

Conclusions:

  • MultiBac is a powerful tool for recombinant multiprotein complex production and metabolic pathway recapitulation.
  • The platform's open-access nature accelerates research in structural biology and drug discovery.
  • Future developments of MultiBac will further expand its applications in academic and industrial R&D.