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Prenylated proteins: the structure of the isoprenoid group
H C Rilling1, E Breunger, W W Epstein
1Department of Biochemistry, University of Utah, Salt Lake City 84112.
Science (New York, N.Y.)
|January 19, 1990
Abstract:
The mevalonate-derived portion of a prenylated protein from Chinese hamster ovary cells has been established as diterpenoid (C20). This group is linked to a carboxyl-terminal cysteine as a thioether. It was removed from the protein by hydrazinolysis followed by Raney nickel desulfurization, and the resulting hydrocarbon fraction was analyzed by gas chromatography-mass spectrometry.