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Updated: May 18, 2026
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
A reaction based turn-on type fluorogenic and chromogenic probe for the detection of trace amount of nitrite in water
Vikash Kumar1, Mainak Banerjee, Amrita Chatterjee
1Department of Chemistry, BITS, Pilani-K.K. Birla Goa Campus, NH 17 B Bypass Road, Zuarinagar, Goa 403726, India.
Abstract:
A turn-on fluorescent probe for the detection of nitrite ion in water is developed based on diazotization reaction of the amino group of the probe in an acidic solution (pH 1). The probe responds selectively to nitrite ion over various other anions with a turn-on type fluorogenic change from colorless to orange by the formation of rhodamine B via an analyte triggered fragmentation process. The fluorescence titration is complete within 1h with 1 equivalent of nitrite ion. The probe is highly efficient, cost-effective and shows a detection limit of 4.6 ppb.
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