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Updated: May 18, 2026

10:01
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Published on: September 27, 2016
Colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity
Wei Shen1, Huimin Deng, Alan Kay Liang Teo
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
Abstract:
Coupling gold nanoparticle-based ligation to a ligation chain reaction enables colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity. One mutant in 2000 copies of a wild-type gene can be detected in 20-100 pg of PCR-amplified genomic DNA samples.

