Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishment and validation of a dual-analyte high-performance liquid chromatography method for detecting thimerosal and phenol in veterinary vaccines.

Scientific reports·2026
Same author

Heterogeneous suppressive effect of Wolbachia incompatible insect technique coupled with sterile insect technique across time and historical Ae. aegypti abundance - using distributional synthetic controls.

PLoS computational biology·2026
Same author

Effects of online collaborative learning and simulation-based learning on pedagogical knowledge and teaching efficacy in digital education.

Scientific reports·2026
Same author

Human-Macaque Contact and Zoonotic Infections in Wild Taiwanese Macaques: Evidence From Southern Taiwan.

American journal of primatology·2026
Same author

Exercise Motivation and Quality of Life in Cancer Survivors: The Impact of Exercise Intervention.

Cancers·2026
Same author

Oral Steroid Use and Asthma Comorbidity in Central Compartment Atopic Disease: The Predictive Role of Olfactory Dysfunction and Eosinophilia.

International forum of allergy & rhinology·2026

Related Experiment Video

Updated: May 18, 2026

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Diagnostic devices for isothermal nucleic acid amplification.

Chia-Chen Chang1, Chien-Cheng Chen, Shih-Chung Wei

  • 1Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan. ccchang.ibme@gmail.com

Sensors (Basel, Switzerland)
|September 13, 2012
PubMed
Summary

Isothermal DNA amplification offers a simpler alternative to polymerase chain reaction (PCR) for genomic analysis. This review covers isothermal methods and recent advancements in diagnostic devices for clinical applications.

Keywords:
HDALAMPNASBARCASDAisothermal nucleic acid amplification

More Related Videos

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification
06:11

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Related Experiment Videos

Last Updated: May 18, 2026

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification
06:11

Rapid Detection of Bacterial Pathogens Causing Lower Respiratory Tract Infections via Microfluidic-Chip-Based Loop-Mediated Isothermal Amplification

Published on: March 29, 2024

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Polymerase Chain Reaction (PCR) enables genomic information retrieval from minimal DNA.
  • PCR requires precise instruments for temperature cycling, limiting routine clinical use.
  • Isothermal approaches offer an alternative by avoiding thermocycling complications.

Purpose of the Study:

  • To describe basic concepts of isothermal DNA amplification.
  • To review recent progress in diagnostic device development for isothermal amplification.

Main Methods:

  • Review of existing literature on isothermal DNA amplification techniques.
  • Analysis of recent advancements in diagnostic devices utilizing isothermal methods.

Main Results:

  • Several isothermal DNA amplification methods are discussed.
  • Recent developments in diagnostic devices for these methods are presented.

Conclusions:

  • Isothermal DNA amplification is a promising area for diagnostic applications.
  • Advancements in diagnostic devices are making isothermal methods more accessible for clinical use.