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Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression
Published on: March 17, 2020
Increased Sox2 copy number in lung squamous cell carcinomas.
Hidefumi Sasaki1, Keisuke Yokota, Yu Hikosaka
1Department of Oncology, Immunology and Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
Experimental and Therapeutic Medicine
|September 13, 2012
Summary
Increased Sox2 gene copy number is common in lung cancer, particularly squamous cell carcinoma (SCC). This finding correlates with gender and smoking status in non-small cell lung cancer patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Sox2 is crucial for foregut, trachea, and lung development.
- Sox2 amplifications have been noted in esophageal and lung cancers.
- The role of Sox2 overexpression in lung cancer progression requires further investigation.
Purpose of the Study:
- To investigate the correlation between Sox2 gene copy number and clinicopathological features in non-small cell lung cancer (NSCLC).
- To determine the prevalence of increased Sox2 gene copy number in NSCLC patients.
Main Methods:
- Real-time polymerase chain reaction (PCR) was used to analyze Sox2 gene copy number in 127 surgically treated NSCLC cases.
- Clinicopathological data including gender, smoking status, and pathological subtype were collected.
- Statistical analysis was performed to identify correlations between Sox2 copy number and patient characteristics.
Main Results:
- An increased Sox2 gene copy number was identified in 33.1% of NSCLC patients.
- Increased Sox2 copy number significantly correlated with male gender (34.1% vs. 9.5% in females, p=0.0026) and smoking status (32.9% in smokers vs. 4.8% in never smokers, p=0.0003).
- Squamous cell carcinoma (SCC) showed a higher prevalence of increased Sox2 copy number (44.8% vs. 7.5% in non-SCC, p<0.0001). Among SCCs, no significant correlation was found with gender, smoking, stage, or differentiation.
Conclusions:
- Increased Sox2 gene copy number is a frequent event in NSCLC, especially in SCC.
- Sox2 copy number alterations may serve as a potential biomarker in lung cancer, particularly in relation to specific subtypes and patient demographics.
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