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Updated: May 18, 2026

The Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat
Published on: June 17, 2016
Sequential analysis of diethylnitrosamine-induced hepatocarcinogenesis in rats
Ryo Abe1, Jun-Ichi Okano, Ryu Imamoto
1Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Tottori 683-8504, Japan.
Abstract:
In the present study, we examined the sequential changes of diethylnitrosamine (DEN)-induced hepatocarcinogenesis in Wistar rats. After 14 weeks of DEN treatment, hyperplastic nodules developed as a consequence of the appearance of renewed hepatocytes, degenerated hepatocytes, oval cells and fibrotic changes. Total bilirubin and alanine aminotransferase levels were significantly higher in the DEN group compared to the control group throughout the experimental period. Our data may prove beneficial to future analyses of chemopreventive compounds during various stages of hepatocarcinogenesis in rats.
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