Mesenchymal stem cell-like cells in classic renal angiomyolipoma

As a benign mesenchymal tumor, classic renal angiomyolipoma (AML) may obliterate the kidney parenchyma and cause renal hemorrhage. It has previously been reported that mesenchymal stem cells (MSCs) are involved in tumorigenesis; however, there have been no studies on stem cells with renal AML origin. In the present study, six females with classic renal AML received a partial or total nephrectomy. During surgery, tumor tissues were collected and culture expansion of adhesive fibroblastoid cells from these tissues was performed. We successfully isolated and cultured MSC-like cells from all six renal AML tumors. MSC characteristics, including morphology, immunophenotype and multidifferentiation potential were analyzed. Flow cytometry analysis revealed that these cells are highly similar to human bone marrow MSCs due to the expression of MSC-specific surface proteins, including CD29, CD44, CD73, CD90 and CD105. The stem cell-like nature of these cells is further supported by their adipogenic and osteogenic differentiation potentials when incubated in appropriate differentiation cocktails. Renal AML-derived adhesive cells possessing the characteristics of MSCs are described for the first time. They are a novel cell type which may be useful in future studies with regards to determining the role of stem cells in the formation and development of renal AML.