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Action Potentials01:41

Action Potentials

Overview

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Related Experiment Video

Updated: May 18, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.

Lauren Barnett1, Jelena Platisa, Marko Popovic

  • 1Department of Cell Biology and Neuroscience, Montana State University, Bozeman, Montana, United States of America.

Plos One
|September 13, 2012
PubMed
Summary

Researchers developed ElectricPk, a novel fluorescent voltage probe for neuroscience. This genetically encoded probe enables faster, more accurate imaging of neural activity and action potentials in specific neurons.

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Related Experiment Videos

Last Updated: May 18, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
06:59

Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes

Published on: September 27, 2018

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biophysics

Background:

  • Genetically encoded fluorescent voltage probes are crucial for studying neural activity in specific neurons and circuits.
  • Existing probes often lack the speed and sensitivity required for real-time monitoring of neuronal action potentials.

Purpose of the Study:

  • To develop a novel, genetically encoded fluorescent voltage probe with enhanced speed and sensitivity for neuroscience applications.
  • To characterize the performance of the new probe in capturing rapid neuronal electrical events.

Main Methods:

  • Constructed 90 variants by fusing the voltage-sensing domain (S1-S4) of Ciona intestinalis voltage-sensitive phosphatase (CiVSP) to circularly permuted eGFP.
  • Tested the developed probe, named ElectricPk, for its response kinetics, fluorescence intensity changes, and linearity with membrane potential shifts.

Main Results:

  • ElectricPk exhibits response kinetics (taus ~1-2 ms), an order of magnitude faster than previously reported fluorescent protein-based voltage probes.
  • The probe accurately tracks neuronal action potentials with a modest fluorescence change (~0.7% ΔF/F).
  • ElectricPk demonstrates a nearly linear response to both hyperpolarizing and depolarizing membrane potential changes.

Conclusions:

  • ElectricPk represents a significant advancement in fluorescent voltage probe technology, offering unprecedented speed for imaging neural activity.
  • The use of circularly permuted fluorescent proteins with voltage-sensing domains shows promise for developing next-generation neural probes.