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Related Experiment Video

Updated: May 18, 2026

Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors
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Battery-triggered microfluidic paper-based multiplex electrochemiluminescence immunodevice based on

Shaowei Wang1, Lei Ge, Yan Zhang

  • 1Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.

Lab on a Chip
|September 14, 2012
PubMed
Summary

This study introduces a battery-powered electrochemiluminescence (ECL) immunoassay on paper devices for multiplex detection of tumor markers. This innovation enables high-throughput, low-cost, and simultaneous analysis for point-of-care diagnostics.

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

  • Analytical Chemistry
  • Biosensors
  • Materials Science

Background:

  • Multiplex electrochemiluminescence (ECL) immunoassays are crucial for high-throughput diagnostics.
  • Microfluidic paper-based analytical devices (μ-PADs) offer low-cost, disposable platforms for point-of-care testing.
  • Traditional μ-PADs often require complex configurations and external equipment like electrochemical workstations.

Purpose of the Study:

  • To develop a potential-resolution strategy for multiplex ECL immunoassay on μ-PADs.
  • To simplify the immunodevice configuration for high-throughput analysis.
  • To enable battery-triggered, constant-potential ECL detection for low-cost, disposable applications, eliminating the need for electrochemical workstations.

Main Methods:

  • Utilized tris-(bipyridine)-ruthenium(ii) (Ru(bpy)(3)(2+)) and carbon nanodots (CNDs) as ECL labels.
  • Developed a simplified immunodevice (μ-PECLI) with two screen-printed carbon electrodes for simultaneous detection of four analytes.
  • Implemented battery-triggered constant-potential ECL detection by controlling electrode potential via connection mode reversal.
  • Designed and fabricated a low-cost voltage controller for precise battery output.

Main Results:

  • Achieved simultaneous detection of four tumor markers using a simplified μ-PECLI device.
  • Demonstrated successful multiplex detection by controlling operational potentials (+1.2 V for Ru(bpy)(3)(2+) and -1.2 V for CNDs) through reversed connections.
  • Validated the assay with human serum samples, showing acceptable agreement with reference values.
  • Successfully performed battery-triggered ECL detection, abandoning the need for an electrochemical workstation.

Conclusions:

  • The developed battery-triggered μ-PECLI offers a novel strategy for high-throughput, low-cost, sensitive, automated, and simultaneous multiplex immunoassays.
  • This approach significantly advances point-of-care diagnostic capabilities.
  • The simplified configuration and battery-powered operation make it suitable for disposable, field-deployable applications.