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Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
Hyperin and quercetin modulate oxidative stress-induced melanogenesis
1Department of Dental Hygiene, Busan Women's College, Busan 617–734, Korea. yjknutr@yahoo.co.kr
Biological & Pharmaceutical Bulletin
|September 14, 2012
Summary
Hyperin and quercetin, natural antioxidants, effectively reduce oxidative stress and melanin synthesis. These compounds show promise in modulating melanogenesis by scavenging reactive species and boosting cellular defense mechanisms.
Area of Science:
- Biochemistry
- Dermatology
- Pharmacology
Background:
- Hyperin and quercetin are phenolic compounds found in fruits and vegetables.
- They possess potent antioxidant properties.
- Their role in mitigating oxidative stress-induced melanogenesis requires further investigation.
Purpose of the Study:
- To investigate the suppressive effects of hyperin and quercetin on oxidative stress.
- To elucidate their impact on melanin synthesis.
- To explore their potential in modulating oxidative stress-induced melanogenesis.
Main Methods:
- In vitro measurement of peroxynitrite (ONOO(-)) scavenging activity.
- Quantification of melanin content, intracellular reactive species (RS), ONOO(-), superoxide ((•)O(2)), and nitric oxide (NO(•)).
- Assay of catalase activity and the reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio in B16F10 melanoma cells.
Main Results:
- Hyperin and quercetin demonstrated in vitro ONOO(-) reduction.
- Both compounds suppressed intracellular RS, ONOO(-), (•)O(2), and NO(•) levels.
- They also reduced melanin synthesis and catalase activity, while increasing the GSH/GSSG ratio in B16 cells.
Conclusions:
- Hyperin and quercetin exhibit significant capacity to scavenge reactive species.
- These compounds effectively suppress oxidative stress markers and melanin production.
- Hyperin and quercetin show potential for modulating oxidative stress-induced melanogenesis.
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